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目的了解2013-2014年本地区副溶血性弧菌的病原学特征,为临床用药提供依据;同时与各地优势脉冲场凝胶电泳(PFGE)型别进行比较,揭示其流行与发展趋势的规律。方法依据GB/T 4789.7-2008进行副溶血性弧菌生化鉴定,同时用荧光定量PCR方法进行复核;采用微量稀释法进行药敏实验;菌株基因组DNA经限制性内切酶Sfi I酶切,用PFGE方法获得电泳图谱,利用Bio Numerics软件对图谱进行同源性分析。结果 88株副溶血性弧菌荧光定量PCR均检出副溶血性弧菌核酸;所有菌株对8种抗生素均敏感;88株菌的DNA指纹图谱可大致分为38种PFGE图谱,经Bio Numerics软件分析分为A~H 8个群,其中F群为优势群。结论荧光定量PCR方法与常规培养法结合是缩短副溶血性弧菌检出时间、提高检出率的有效方法;从大连市患者粪便中检出的副溶血性弧菌对临床常用的8种抗生素尚无耐药情况且亲缘关系较近。
Objective To understand the etiological characteristics of Vibrio parahaemolyticus in the region from 2013 to 2014, and to provide a basis for clinical drug use. The prevalence and development trend of Vibrio parahaemolyticus in different regions were also compared with those of other PFGE genotypes. Methods The biochemical identification of Vibrio parahaemolyticus was carried out according to GB / T 4789.7-2008, and the results were checked by real-time quantitative PCR. The drug susceptibility test was carried out by using the microdilution method. The genomic DNA of the strain was digested with restriction endonuclease Sfi I PFGE method to obtain electrophoresis patterns, using Bio Numerics software homology analysis of the map. Results 88 strains of Vibrio parahaemolyticus were detected by fluorescence quantitative PCR. All strains were sensitive to 8 kinds of antibiotics. The DNA fingerprinting profiles of 88 strains were divided into 38 PFGE profiles, and were analyzed by Bio Numerics software Analysis is divided into A ~ H 8 groups, of which F group is the dominant group. Conclusion Fluorescence quantitative PCR method combined with routine culture method is an effective method to shorten the detection time of Vibrio parahaemolyticus and increase the detection rate. Vibrio parahaemolyticus detected in feces of Dalian patients is very helpful to the detection of 8 commonly used antibiotics No drug resistance and close relatives.