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【目的】Wolbachia是广泛存在于节肢动物体内的一类母系遗传的共生菌,能够通过多种机制调节节肢动物的生殖。近年来,为了更进一步地探究Wolbachia与寄主之间的互作机制,许多研究者展开了Wolbachia的人工转染研究。【方法】我们在实验室条件下将灰飞虱Laodelphax striatellus(Fallén)感染的Wolbachia提取纯化后,利用显微注射的方法导入豆叶螨Tetranychus phaselus Ehara体内。研究了注入从灰飞虱提取的Wolbachia和豆叶螨自然感染Wolbachia对豆叶螨繁殖适合度和寿命的影响,并测定了两种Wolbachia的密度随豆叶螨日龄增长的变化情况。【结果】结果显示,外源Wolbachia在豆叶螨体内的拷贝数极低,仅为自然感染豆叶螨体内Wolbachia拷贝数的0.5%左右。与自然感染的Wolbachia不同,外源Wolbachia在豆叶螨种群中不能引起胞质不亲和,但能够显著降低雌螨的产卵量。【结论】本研究表明,来自灰飞虱的Wolbachia具有抑制豆叶螨种群扩张的潜在能力,对豆叶螨生物防治具有一定的应用价值。
【Objective】 Wolbachia is a kind of maternally inherited symbiotic bacteria widely distributed in arthropods and regulates the reproduction of arthropods through various mechanisms. In recent years, in order to further explore the mechanism of Wolbachia and host interaction, many researchers carried out Wolbachia artificial transfection studies. 【Method】 We extracted and purified Wolbachia infected with Laodelphax striatellus (Fallén) under laboratory conditions and introduced them into Tetranychus phaselus Ehara by microinjection. The effects of natural Wolbachia and Bemisia tabaci extracted from Laodelphax striatellus and Wolbachia on the reproductive fitness and longevity of Bemisia tabaci were studied. The changes of the two Wolbachia ’s densities with the age were also studied. 【Result】 The results showed that the copy number of exogenous Wolbachia in the spider mite body was extremely low, which was only about 0.5% of the Wolbachia copy number of the naturally infected spider mite body. Unlike naturally infected Wolbachia, exogenous Wolbachia does not cause cytoplasmic incompatibility in the Bemisia tabaci population, but can significantly reduce female fecundity. 【Conclusion】 This study shows that Wolbachia from Laodelphax striatellus has the potential to inhibit the population expansion of bean spider mite and has certain value for the biological control of bean spider mite.