近年来,大量采用体外细胞培养技术研究垂体细胞分泌功能。原代培养垂体细胞通常采用添加血清或组织抽提物的培养液,血清通过提供营养物、激素、载体蛋白和各种生长调节因子促进细胞新陈代谢,尤其对垂体细胞的发育和分化、受体的诱导及表达、激素或神经递质的产生均具有重要作用.但血清成分复杂,其中存在的未知因子可能使实验结果混乱。本实验采用无血清原代培养大鼠垂体前叶和中/后叶细胞,细胞收获量大,形态完整,分泌功能正常。 In recent years, a large number of in vitro cell culture techniques to study the secretion of pituitary cells. Primary cultured pituitary cells are usually cultured in serum or tissue extracts. Serum promotes cell metabolism by providing nutrients, hormones, carrier proteins and various growth regulators, especially for the development and differentiation of pituitary cells, Induction and expression, hormones or neurotransmitters have an important role.But the serum composition is complex, the presence of unknown factors may make the experimental results confusion. In this experiment, serum-free primary cultured rat anterior pituitary and middle / posterior lobe cells, the harvest of large cells, complete morphology, secretion of normal.