近年来,阳离子聚合物、阳离子脂质体等非病毒载体在基因递送中的应用越来越受到人们的关注.然而,血清的存在会降低它们的转染效率,从而阻碍它们在临床中的使用.因此,非常有必要开发能抵抗血清干扰的非病毒基因载体.本工作合成了一种新型的锌离子配位的脂质,其与1,2-二油酰-S N-甘油-3-磷酰乙醇胺脂质(DOPE)共自组装形成阳离子脂质体(Zn-DTAc脂质体).实验结果表明,Zn-DTAc脂质体可以有效地压缩质粒形成纳米复合物.即使在50％胎牛血清存在的情况下,该复合物也能进行有效的细胞内化和内涵体逃逸,并实现高效的基因转染.“,”In recent years,non-viral vectors,such as cationic polymers and liposomes,have attracted increasing attentions for their applications in gene delivery.However,reduced transfection efficiency inhibits their further use in the presence of serum.Therefore,it is necessary to develop serum-tolerant non-viral gene vectors.Herein,a novel Zn coordinated lipid is synthesized,which self-assembles with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine lipid ( DOPE ),forming cationic liposome ( Zn-DTAc liposome).The experimental results demonstrate that Zn-DTAc liposomes can effectively condense pDNA into nanoparticles,which have high cellular uptake and effective endosomal escape and exhibit efficient gene transfection even in the presence of 50％ fetal bovine serum.