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目的：观察中脑楔状核（ＮＣＦ）尾部给予外源性吗啡对延髓中缝大核（ＮＲＭ）和中脑导水管周围灰质（ＰＡＧ）内痛兴奋神经元（ＰＥＮ）的痛放电影响。方法：用玻璃微电极细胞外记录方法，分别在ＮＲＭ和ＰＡＧ内记录ＰＥＮ的单位放电。结果：（１）ＮＣＦ内注入吗啡（３×１０－２ｍｏｌ／Ｌ）能明显抑制ＰＡＧ、ＮＲＭ内ＰＥＮ的痛放电频率；（２）ＮＣＦ内微量注入纳洛酮（２．７４×１０－３ｍｏｌ／Ｌ）能增加ＰＡＧ、ＮＲＭ内ＰＥＮ的痛放电频率；（３）ＮＣＦ内预先注射纳洛酮（５．５×１０－３ｍｏｌ／Ｌ）能部分阻断吗啡对ＰＡＧ、ＮＲＭ内ＰＥＮ痛放电抑制效应。结论：ＮＣＦ内某些神经元与抗伤害性感受有关；吗啡有可能是ＮＣＦ抗伤害感受作用中的递质或调质
OBJECTIVE: To observe the effect of exogenous morphine given to the tail of midbrain wedge nucleus (NCF) on painful discharge of painful excitatory neurons (PEN) in medulla oblongata (NRM) and periaqueductal gray (PAG). METHODS: Extracellular recordings of glass microelectrodes were used to record the unit discharge of PEN in NRM and PAG, respectively. Results: (1) Morphine (3 × 10-2mol / L) injected into NCF could significantly inhibit the pain frequency of PEN and PEN in NRM; (2) Naloxone (2.74 × 10-3mol / (P <0.05). (3) Pretreatment with naloxone (5.5 × 10-3mol / L) in NCF partially blocked the inhibitory effect of morphine on PEN pain in PAG and NRM . CONCLUSIONS: Some neurons in NCF are associated with antinociceptive effects; morphine may be a neurotransmitter or quencher in NCF antinociception