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目的:通过体外树突状细胞(dendritic cells，DCs)试验和体内小鼠免疫试验探究新疆野生一枝蒿醇提物(ethanol extract of wild n Artemisia rupestris L．，EEWAR)的免疫调节活性和安全性。n 方法:体外试验中，采用不同剂量EEWAR体外刺激C57BL/6小鼠骨髓来源树突状细胞(bone marrow dendritic cells，BMDCs)，流式细胞术检测BMDCs表面分子CD40和CD80的表达。体内试验中，选取不同剂量EEWAR与模式抗原卵清蛋白(ovalbumin，OVA)皮下免疫小鼠，以铝佐剂为对照，检测免疫后小鼠血清中OVA特异性IgG及分型抗体水平，MTT法观察脾脏中淋巴细胞增殖水平。同时，选取不同剂量EEWAR免疫ICR小鼠进行动物急性毒性试验评价其安全性。结果:体外试验表明，EEWAR在10～20 μg/ml的剂量范围内能显著促进BMDCs表面分子的表达(n P<0.05)，且对BMDCs形态影响不大；在100～200 μg/ml的剂量范围内能极显著促进BMDCs表面分子CD40和CD80的表达(n P<0.01)，但对BMDCs形态有一定影响。体内试验表明，EEWAR能极显著增强OVA特异性IgG及分型抗体水平(n P<0.01)，并显著促进淋巴细胞增殖(n P<0.05)。急性动物试验表明，50～5 000 mg/kg的EEWAR对小鼠体重没有影响，具有一定的安全性。n 结论:EEWAR能够刺激DCs成熟，作为OVA的佐剂可以提高体液和细胞免疫水平，具有一定的安全性。本研究为EEWAR作为新型佐剂的候选资源研究提供参考。“,”Objective:To evaluate the n in vitro and n in vivo immunostimulatory activity and the safety of ethanol extract of wild n Artemisia rupestris L. (EEWAR).n Methods:Bone marrow dendritic cells (BMDCs) from C57BL/6 mice were treated with different concentrations of EEWAR n in vitro and the expression of CD40 and CD80 on BMDCs was detected by flow cytometry. ICR mice were subcutaneously immunized with different concentrations of EEWAR in combination with ovalbumin (OVA) or OVA alone. Aluminum adjuvant was used as the positive control. OVA-specific IgG antibodies in mouse serum samples were measured by ELISA following immunization. T cell proliferation in spleen tissues was detected by MTT method. Acute toxicity test was conducted in ICR mice to analyze the safety of EEWAR.n Results:In vitro experiment showed that EEWAR at the concentrations of 10-20 μg/ml increased the expression of CD40 and CD80 on BMDCs ( n P<0.05), and had no significant effect on the morphology of BMDCs; EEWAR at the concentrations of 100-200 μg/ml significantly promoted the expression of CD40 and CD80 on BMDCs (n P<0.01), but had a certain influence on the morphology of BMDCs.n In vivo experiment showed that EEWAR enhanced the production of IgG, IgGn 1 and IgGn 2a antibodies against OVA and the proliferation of splenocytes (n P<0.05). In the acute toxicity test, EEWAR at the concentrations of 50-5 000 μg/ml had no side effects on mouse body weight and was relatively safe.n Conclusions:EEWAR could promote the maturation of DCs and enhance the humoral and cellular immune responses when used as an adjuvant to OVA. It was safe in a certain dose range. This study provided reference for further research on EEWAR as a new-generation adjuvant.