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用纸电泳法分离检测果糖二磷酸钠中有关物质６磷酸果糖及果糖，分离效果好，方法简便快速，检出灵敏度高。以枸橼酸盐缓冲液（ｐＨ３０）为电泳缓冲液，采用中速薄型定量层析滤纸作电泳滤纸，电压梯度１８Ｖ／ｃｍ，电泳８０ｍｉｎ，用盐酸间苯二胺乙醇液显色。以果糖二磷酸钠泳动距离为１００，６磷酸果糖和果糖的相对迁移率（ｎ＝１２）分别为０７４±００６、０１２±００３。最低检出量分别为果糖二磷酸钠２μｇ，６磷酸果糖５μｇ，果糖０２５μｇ。
Paper Electrophoresis separation and detection of fructose diphosphate in the relevant substances 6-phosphate fructose and fructose, the separation effect is good, the method is simple and fast, the detection of high sensitivity. Using citrate buffer (pH30) as electrophoresis buffer and medium-speed thin quantitative chromatography filter paper as electrophoresis filter, the voltage gradient was 18V / cm and the electrophoresis was performed for 80min. The relative mobility of fructose-fructose and fructose was 074 ± 006 and 012 ± 003, respectively. The lowest detection rates were 2μg of fructose diphosphate, 6μg of fructose 5μg, fructose 0 25μg.