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目的 观察哮喘大鼠肺组织中诱导型一氧化氮合酶 (i NOS)活性的表达 ,探讨一氧化氮在哮喘大鼠气道炎症中的作用。方法 用卵白蛋白作为致敏原制备哮喘大鼠模型 ,用 SP免疫组化染色方法检测肺组织 i NOS的表达并观察 i NOS在气道组织分布的改变。结果 哮喘大鼠肺组织 i NOS的表达阳性率 (90 % )明显高于正常对照组 (2 0 % ) (P<0 .0 0 1)。哮喘大鼠气道组织 i NOS表达阳性细胞主要位于气道上皮细胞、气道平滑肌细胞、血管内皮和平滑肌细胞、浸润的中性粒细胞和巨噬细胞 ,而淋巴细胞表达不明显。用甲基强的松龙处理后哮喘大鼠肺组织i NOS表达阳性率 (30 % )明显降低。结论 以上结果提示一氧化氮在哮喘气道炎症中起重要作用 ,用甲基强的松龙治疗哮喘可以使哮喘大鼠肺组织中 i NOS表达阳性率降低 ,提示哮喘时产生过多的一氧化氮可能有加重气道炎症的作用
Objective To observe the expression of inducible nitric oxide synthase (iNOS) in lung tissue of asthmatic rats and to explore the role of nitric oxide in airway inflammation in asthmatic rats. Methods Asthma rat model was established by using ovalbumin as sensitizer. The expression of iNOS in lung tissue was detected by SP immunohistochemistry and the distribution of iNOS in airway was observed. Results The positive rate of iNOS expression in the lung tissue of asthmatic rats was significantly higher than that of the normal control group (90%) (P <0.01). The positive expression of i NOS in airway of asthmatic rats was mainly located in airway epithelial cells, airway smooth muscle cells, vascular endothelium and smooth muscle cells, infiltrating neutrophils and macrophages, while the expression of lymphocytes was not obvious. The positive rate (30%) of iNOS expression in lung tissue of asthmatic rats was significantly decreased after treatment with methylprednisolone. Conclusion The above results suggest that nitric oxide plays an important role in the airway inflammation in asthma. Treatment of asthma with methylprednisolone can reduce the iNOS expression in the lung tissue of asthmatic rats, suggesting that excessive production of nitric oxide Nitrogen may have an aggravating effect on airway inflammation