论文部分内容阅读
目的观察锌过量对幼鼠脑发育和学习记忆行为的影响。方法从哺乳期开始建立过量锌幼鼠模型，采用电镜观察、神经生化和神经行为观察等方法。结果随饲料锌含量的增加幼鼠大脑及海马重量减少，血清及海马锌增高。过量锌１、２组（ＺＥ１、ＺＥ２组）幼鼠主动回避反应（ＡＡＲ）习得率及活动明显低于自由喂养组（ＡＬ组）及ＺＥ２组的配对组（ＰＦ组），ＺＥ２组的恢复组（ＺＥ２Ｒ组）ＡＡＲ习得率也明显低于ＡＬ和ＰＦ组，但明显高于ＺＥ２组。ＰＦ组与ＡＬ组ＡＡＲ习得率差异无显著性，但活动较ＡＬ组多。ＺＥ２组海马一氧化氮含量增加而生长抑素含量减少，脑组织兴奋性氨基酸和抑制性氨基酸总量增高，尤其是谷氨酸增高更为明显。ＺＥ２组海马ＣＡ１区神经元、胶质细胞及细胞器均有不同程度变性、坏死，突触小泡破裂。结论过量锌饲料明显影响幼鼠脑发育和功能，其机理是多方面的
Aim To observe the effects of zinc overdose on brain development, learning and memory in young rats. Methods From the beginning of lactation, an excessive zinc young rat model was established and observed by electron microscopy, neurobiology and neurobehavioral observation. Results With the increase of dietary zinc, the weight of brain and hippocampus in young rats decreased and the serum zinc and hippocampus increased. The acquisition rate and activity of active avoidance reaction (AAR) in young Zinc group 1, 2 group (ZE1, ZE2 group) were significantly lower than that in free group (AL group) and ZE2 group (PF group), ZE2 group Group A (ZE2R group) AAR acquisition rate was significantly lower than the AL and PF group, but significantly higher than the ZE2 group. There was no significant difference in AAR acquisition rate between PF group and AL group, but there were more activities than AL group. In the ZE2 group, the content of nitric oxide increased while the content of somatostatin decreased. The total amount of excitatory amino acids and inhibitory amino acids in brain increased, especially the glutamate increased obviously. ZE2 group of hippocampal CA1 area neurons, glial cells and organelles have varying degrees of degeneration, necrosis, synaptic vesicle rupture. Conclusion Excessive zinc diets significantly affect brain development and function in young rats, and its mechanism is multifaceted