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目的研究血管紧张素Ⅱ2型受体(AT2R)基因A1675G多态性与山东济宁地区汉族女性原发性高血压(EH)的相关性。方法随机选取山东省济宁地区汉族女性EH患者127例(EH组)和体检正常的女性119例(正常对照组),提取外周血基因组DNA,PCR扩增A1675G基因,高分辨率熔解(HRM)法测定A1675G基因的多态性。结果EH组AA、AG和GG基因型频率(分别为26.8%、53.5%和19.7%)与正常对照组(分别为27.7%、42.0%和30.3%)比较,差异均无统计学意义;等位基因频率(A:53.5%;G:46.5%)与正常对照组(A:48.7%;G:51.3%)比较,差异也无统计学意义。结论A1675G基因多态性可能与山东省济宁地区汉族女性原发性高血压无明显相关性。
Objective To investigate the association between A1675G polymorphism of angiotensin Ⅱ type 2 receptor (AT2R) gene and essential hypertension (EH) in Han women in Jining, Shandong Province. Methods A total of 127 EH patients (EH group) and 119 healthy women (normal control group) were enrolled in this study. The genomic DNA of peripheral blood was extracted and the A1675G gene was amplified by PCR. High resolution melting (HRM) Determination of A1675G gene polymorphism. Results The frequencies of AA, AG and GG genotypes in EH group (26.8%, 53.5% and 19.7%, respectively) were not significantly different from those in normal controls (27.7%, 42.0% and 30.3%, respectively) The gene frequency (A: 53.5%; G: 46.5%) compared with the normal control group (A: 48.7%, G: 51.3%), the difference was not statistically significant. Conclusion The polymorphism of A1675G gene may not be associated with the primary hypertension in Han nationality in Jining of Shandong Province.