论文部分内容阅读
目的:研究p16INK4A和p57KIP2在宫颈脱落细胞的表达及其与临床病理指标的关系。方法:采用免疫细胞化学二步法,检测81例不同病变阶段的宫颈脱落细胞标本中p16INK4A和p57KIP2的表达。结果:p16INK4A在ASC-US、低级别上皮内病变(CINⅠ)、高级别上皮内病变(CINⅡ～Ⅲ)和浸润性癌的阳性表达率分别为6.25%(1/16)、58.8%(10/17)、89.2%(33/37)和100.0%(11/11),各组之间的差异有统计学意义,χ2=38.806,P<0.001;p57KIP2在高级别上皮内病变(CINⅡ～Ⅲ)和浸润性癌的阳性表达率为27.1%(13/48),与ASC-US和低级别上皮内病变(CINⅠ)的阳性表达率为69.7%(23/33)比较,差异有统计学意义,χ2=15.194,P=0.002。结论:p16INK4A和p57KIP2在宫颈脱落细胞中的表达与临床病理指标密切相关,p16INK4A和p57KIP2可以作为宫颈癌前病变高危人群的筛查指标。
Objective: To study the expression of p16INK4A and p57KIP2 in cervical exfoliated cells and its relationship with clinicopathological parameters. Methods: Immunocytochemical two-step method was used to detect the expression of p16INK4A and p57KIP2 in 81 specimens of cervical exfoliated cells with different pathological stages. Results: The positive rates of p16INK4A in ASC-US, CINⅠ, CINⅡ-Ⅲ and invasive carcinoma were 6.25% (1/16) and 58.8% (10 / 17), 89.2% (33/37) and 100.0% (11/11) respectively. There was a significant difference between the groups (χ2 = 38.806, P <0.001) And invasive carcinoma (27.1%, 13/48), respectively. The positive rates of ASC-US and low-grade intraepithelial neoplasia (CINⅠ) were 69.7% (23/33), the difference was statistically significant χ2 = 15.194, P = 0.002. Conclusion: The expression of p16INK4A and p57KIP2 in cervical exfoliated cells is closely related to the clinicopathological parameters. P16INK4A and p57KIP2 can be used as the screening index for cervical precancerous lesions.