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目的分析DNA甲基转移酶3B(DNMT3B)基因启动子区-579G>T单核苷酸多态性(SNP)与江苏宿迁地区汉族人群胃癌(GC)易感性的关系。方法病例组为233例胃癌患者,对照组为208例体检健康者。提取外周血基因组DNA,利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测DNMT3B启动子区-579G>T位点的基因频率分布。结果 TT基因型与GT+GG基因型频率在胃癌组和对照组中差异有统计学意义(χ~2=6.517,P<0.05)。按年龄分组后,年龄≥60岁的胃癌组和对照组人群间比较,TT与GT+GG基因型频率差异有统计学意义(χ~2=4.728,P<0.05)。按性别分组后,2组间基因型频率差异均无统计学意义(χ~2=3.541,P>0.05;χ~2=3.676,P>0.05)。结论 DNMT3B-579G>T与胃癌的遗传易感性有关联,可作为胃癌遗传易感性风险因素。
Objective To analyze the relationship between -579G> T SNP in promoter region of DNA methyltransferase 3B (DNMT3B) and susceptibility to gastric cancer (GC) in Han population in Suqian, Jiangsu Province. Methods 233 cases of gastric cancer patients, control group of 208 cases of healthy people. Peripheral blood genomic DNA was extracted and the gene frequency distribution at -579G> T site of DNMT3B promoter region was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There was significant difference between TT genotype and GT + GG genotype in gastric cancer group and control group (χ ~ 2 = 6.517, P <0.05). The frequency of TT and GT + GG genotypes was significantly different between groups of gastric cancer ≥60 years old and controls (χ ~ 2 = 4.728, P <0.05) after being grouped by age. There were no significant differences in genotype frequencies between the two groups (χ ~ 2 = 3.541, P> 0.05; χ ~ 2 = 3.676, P> 0.05). Conclusion DNMT3B-579G> T is associated with the genetic susceptibility to gastric cancer and may be used as a risk factor for genetic susceptibility to gastric cancer.