论文部分内容阅读
简述了当今植物抗虫基因工程的研究进展 ,介绍了Bt基因和蛋白酶抑制剂基因 ,农杆菌介导的遗传转化以及防治害虫产生抗性的策略等。双抗虫基因是用部分改造BtCry1Ac基因和慈菇蛋白酶抑制剂基因 (API)构建的植物表达载体。被转化体 74 1杨 (Populus×aldatomen tosaCl.74 1)是以银白杨× (山杨 +小叶杨 )为母本 ,毛白杨为父本 ,1994年杂交育成 ,为一优良白杨无性系 ,形态酷似毛白杨。在转双抗虫基因的研究中 ,选出高抗虫、中抗虫无性系。高抗虫无性系杀死昆虫幼虫的总死亡率为 83%～ 90 %。从苗期高生长和形态观察 ,转双抗虫基因 74 1杨生长发育正常。比较详细地介绍了 74 1杨营养繁殖技术及基本原理。介绍了硬枝扦插容器育苗、组培微繁技术、容器育苗的移栽和苗圃管理以及采穗圃的建立和经营等
In this paper, the research advances of plant pest-resistance gene engineering are briefly introduced. The genes of Bt and protease inhibitors, Agrobacterium-mediated genetic transformation and the strategies of controlling pest resistance are introduced. The double-pest-resistant gene is a plant expression vector partially engineered with the BtCry1Ac gene and the arrowhead protease inhibitor gene (API). Populus × aldatomen tosaCl.74 1 was a good Poplar × Populus davidiana × Populus × Populus × Populus trichocarpa and Populus tomentosa male parent. Similar to the white poplar. In the double transgenic insect resistance genes, selected high insect resistance, insect resistance clones. The total mortality of highly resistant clones killing insect larvae was 83% to 90%. From the high growth and morphological observation at the seedling stage, the growth and development of the double insect-resistant gene 741 was normal. Introduced 74 1 Yang vegetative propagation technology and basic principle in more detail. Introduced the hardwood cutting container seedling, tissue culture micropropagation technology, container seedling transplanting and nursery management, as well as the establishment and management of sprouts nursery