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向培养液中加入15mmol／L的β-AP1-40，48h后，发现原代培养的海马细胞内Ca2+的浓度明显增高，对照组为23．9±3．6（n=51）而β-AP组为36．5±15．8（n=30），两组比较P＜0．001，有非常显著性的差别。对照组的乳酸脱氢酶没有明显变化，而β－AP组在加β－AP1－4048h后乳酸脱氢酶明显增加，从52iu／L增加到105iu／L，实增加了101％。实验结果表明，β－AP1-40对培养的神经细胞有明显的毒性作用，但没有发现形态学的变化和对细胞的特异性。如在培养液中加β－AP1-40的同时再加25mg／ml的神经节苷脂（GAs）可明显减弱β-AP1-40引发的胞内Ca2+浓度和培养液中LDH浓度的升高。β-AP＋GAs组胞内Ca2+浓度为27.7±5.58，与β-AP组比较，有显著性差异（P＜0．01）。β-AP组海马细胞培养液中乳酸脱氢酶的浓度增高为101％，而β-AP-GAs组培养液中乳酸脱氢酶的浓度增高仅为67％。在NG108－15细胞上也获得了相同的结果。单唾液酸神经节苷脂虽有与GAs类似的作用，但较弱。这些实验结果指出，神经节苷脂和单唾液酸神经节苷脂均能拮抗β-AP1-40的毒性作用，从而对细胞可起到保护作用。
The concentration of Ca2 + in primary cultured hippocampal cells was significantly increased after adding 15mmol / L β-AP1-40 for 48h to the culture medium, the control group was 23.9 ± 3.6 (n = 51) AP group was 36.5 ± 15.8 (n = 30), the two groups were P <0.001, there is a very significant difference. Lactate dehydrogenase in the control group did not change significantly, while in the β-AP group, lactate dehydrogenase increased significantly from 52iu / L to 105iu / L after adding β-AP for 1-4048h, an increase of 101%. The experimental results showed that β-AP1-40 had obvious toxic effects on cultured nerve cells, but no morphological changes and cell specificity were found. Adding β-AP1-40 and 25mg / ml gangliosides (GAs) to culture medium could significantly reduce the intracellular Ca2 + concentration induced by β-AP1-40 and the increase of LDH concentration in culture medium. The intracellular Ca2 + concentration in β-AP + GAs group was 27.7 ± 5.58, which was significantly different from that in β-AP + GAs group (P <0.01). The concentration of lactate dehydrogenase in hippocampal cell culture fluid of β-AP group was increased by 101%, while the concentration of lactate dehydrogenase in β-AP-GAs group culture medium was only increased by 67%. The same result was also obtained on NG108-15 cells. Monosialogangliosides, although similar to GAs, are weaker. These experimental results indicate that both gangliosides and monosialogangliosides can antagonize the toxic effects of β-AP1-40 and thus have a protective effect on the cells.