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目的了解宁波口岸进、出口海产品中副溶血性弧菌的整合子和毒力基因分布情况。方法将来自宁波口岸海产品中分离的120株副溶血性弧菌作为研究对象,利用PCR技术检测其整合子和6个毒力基因(tdh、tlh、trh、toxR、Ure C和T3SS2)携带率。结果 120株副溶血性弧菌中,Ⅰ类整合子总携带率为34.2%、出口海产品中的携带率为31.7%、进口海产品中的携带率为36.7%,Ⅱ类和Ⅲ类整合子都为阴性;毒力基因tlh、toxR的携带率为100%,Ure C的携带率为4.17%、T3SS2的携带率为5.83%、tdh的携带率为2.50%,trh全部阴性。结论进、出口海产品中副溶血性弧菌存在携带毒力基因的风险不高,进口和出口海产品中毒力基因携带率差异很小,整合子携带率高,但对食品安全具有潜在的威胁,仍需要加强监测。
Objective To understand the distribution and virulence genes of Vibrio parahaemolyticus in imported and exported seafood in Ningbo Port. Methods 120 strains of Vibrio parahaemolyticus isolated from the seafood of Ningbo Port were taken as research objects. The PCR products were used to detect the rate of carriage of six virulence genes (tdh, tlh, trh, toxR, Ure C and T3SS2) . Results Among the 120 strains of Vibrio parahaemolyticus, the total carriage rate of class Ⅰ integrons was 34.2%, that of export products was 31.7%, that of imported products was 36.7%, that of class Ⅱ and Ⅲ integrons All were negative; virulence genes tlh, toxR carrying rate of 100%, Ure C carrying rate of 4.17%, T3SS2 carrying rate of 5.83%, tdh carrying rate of 2.50%, trh all negative. Conclusions The risk of virulence genes carrying Vibrio parahaemolyticus in imported and exported seafood is not high, the difference of carrying rate of virulence genes in imported and exported seafood is very small, and the carry rate of integron is high, but it is a potential threat to food safety , There is still a need to strengthen monitoring.