本文概述了当前肿瘤基因治疗研究中存在的一些主要问题 ,如绝大多数治疗方案中目的基因只有一个 ,肿瘤基因治疗缺乏靶向性 ,基因转移载体的效率、安全性及容量等问题。讨论了解决这些问题的主要途径 ,即肿瘤多基因联合治疗、直接体内途径基因治疗与靶向基因治疗、基因转移载体的改造。 This article outlines some of the major problems in current cancer gene therapy research. For example, there are only one gene of interest in most treatment protocols, lack of targeting of tumor gene therapy, efficiency, safety, and capacity of gene transfer vectors. Discussed the main ways to solve these problems, namely tumor multi-gene therapy, direct in vivo pathway gene therapy and targeted gene therapy, gene transfer vector transformation.