论文部分内容阅读
目的:探讨转甲状腺素(TTR)蛋白含量和化学修饰类型在诊断肺感染性疾病中的作用。方法:(1)测定22例肺感染患者血清和胸腔积液中总蛋白(TP)、白蛋白(ALB)、三酰甘油(TG)、胆固醇(Cho)、载脂蛋白(Apo)A、ApoB、乳酸脱氢酶(LDH)、腺苷脱氨酶(ADA)、葡萄糖(GLU)和TTR等指标水平。(2)基质辅助激光电离飞行时间质谱(MALDI-TOF-MS)分析肺感染患者血清和胸腔积液TTR蛋白的化学修饰;作为对照测定16例健康人血清TTR的修饰。结果:(1)胸腔积液TG、Cho、TTR、ApoA、ApoB、TP、ALB和GLU指标水平均明显低于血清,仅ADA指标增高,差异有统计学意义(P<0.05或P<0.01)。(2)蛋白指标中TTR减低最明显。(3)肺感染患者胸腔积液和血清中只发现两种修饰类型,而无对照组的cysgly-TTR修饰型。结论:血清及胸腔积液中TTR含量和cysgly-TTR的减少,可能作为辅助诊断肺感染指标。
Objective: To investigate the role of transthyretin (TTR) protein content and chemical modification in the diagnosis of pulmonary infectious diseases. Methods: (1) The levels of total protein (TP), albumin (ALB), triglyceride (TG), cholesterol, apolipoprotein A , Lactate dehydrogenase (LDH), adenosine deaminase (ADA), glucose (GLU) and TTR and other indicators. (2) Chemical modification of TTR protein in sera and pleural effusion of patients with lung infection by matrix-assisted laser ionization time of flight mass spectrometry (MALDI-TOF-MS); TTR as a control was measured in 16 healthy volunteers. Results: (1) The levels of TG, Cho, TTR, ApoA, ApoB, TP, ALB and GLU in pleural effusion were significantly lower than those in serum and only the ADA index was increased (P <0.05 or P <0.01) . (2) The most obvious reduction of TTR in protein index. (3) There were only two types of pleural effusion in serum and serum of patients with lung infection, but no cysgly-TTR modification in control group. Conclusion: The reduction of TTR and cysgly-TTR in serum and pleural effusion may be used as an auxiliary diagnostic indicator of lung infection.