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目的:研究基质金属蛋白酶9(MMP-9)基因多态性与缺血性脑卒中(IS)发病及预后的相关性,为IS的防治提供新的理论依据。方法:选取治疗的IS患者100例,根据TOAST分型标准分为大动脉粥样硬化型(LAA)组41例,小动脉阻塞型(SAO)组59例,并选取健康体检者40例作为对照组,采用PCR-RFLP法检测各组MMP-9基因C1562T、R279Q多态性,并对IS患者进行3个月的随访,采用Logistic回归分析C1562T、R279Q多态性与IS患者预后的相关性。结果:LAA组、SAO组MMP-9基因C1562T位点T等位基因、C/T+T/T基因型频数均高于对照组,差异有统计学意义(P<0.05),LAA组、SAO组C1562T位点C等位基因、C/C基因型频数及R279Q位点等位基因和基因型频数与对照组比较差异无统计学意义(P>0.05);Logistic回归分析显示,MMP-9各型别基因与预后无明显相关性(P>0.05)。结论:MMP-9基因C1562T的T等位基因是IS发病的穿易感基因之一,但MMP-9基因多态性与IS患者的预后并无明显相关性。
Objective: To investigate the relationship between the polymorphism of matrix metalloproteinase 9 (MMP-9) and the onset and prognosis of ischemic stroke (IS), and to provide a new theoretical basis for the prevention and treatment of IS. Methods: One hundred patients with IS were selected according to the criteria of TOAST, including 41 patients with large atherosclerosis (LAA) and 59 patients with small artery occlusion (SAO). 40 healthy subjects were selected as the control group PCR-RFLP was used to detect the C1562T and R279Q polymorphisms of MMP-9 gene in each group. The IS patients were followed up for 3 months. Logistic regression was used to analyze the correlation between C1562T and R279Q polymorphisms and the prognosis of IS patients. Results: The frequency of T allele and C / T + T / T genotype of C1562T in LAA group and SAO group were significantly higher than those in control group (P <0.05) There was no significant difference in C allele, C / C genotype frequency and R279Q locus allele and genotype frequency between C1562T group and control group (P> 0.05). Logistic regression analysis showed that the expression of MMP-9 There was no significant correlation between genotype and prognosis (P> 0.05). CONCLUSION: The T allele of C1562T is one of the predisposing genes of IS. However, there is no significant correlation between the polymorphism of MMP-9 and the prognosis of patients with IS.