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目的:探究不同时辰对阳明腑实证模型SD大鼠灌胃大承气汤的形态学变化,以取得最佳疗效的服药时辰。方法:将70只SD大鼠,随机分为辰时(8点)组、巳时(10点)组、午时(12点)组、未时(14点)组、申时(16点)组、酉时(18点)组和对照组。造模后分别于各时辰点以大承气汤(19g/kg)灌胃,观察给药前后的体温、腹围以及胃、结肠病理形态学变化。结果:①体温:造模后各实验组体温均升高(P<0.05)。在灌胃大承气汤后,实验组与造模后比较体温下降(P<0.05)。灌药后午时组体温高于辰时组、巳时组(P<0.05),未时组、申时组、酉时组体温均高于辰时组(P<0.05)。②腹围:与对照组比较,造模后各实验组腹围均缩小(P<0.05),大承气汤灌胃后,各实验组腹围均缩小,其中辰时组缩小最明显(P<0.05)。各时辰点灌药后形态学存在差异,以辰时(8点)组形态学变化最明显,午时(12点)组变化最小,其他各组介于2组之间。结论:不同时辰给药的形态学变化存在差异,在白天的6个时辰中以辰时服药变化最显著,午时变化最不明显,其余时辰变化介于上述两者之间。
OBJECTIVE: To investigate the morphological changes of Dachengqi Decoction at different time points in Yangmingfu experimental model SD rats in order to obtain the best curative time. Methods: Seventy SD rats were randomly divided into three groups: the control group (8 points), the control group (10 points), the noon (12 points) group, the no time (14 points) 18 points) group and control group. After modeling, Dachengqi Decoction (19g / kg) was intragastrically administered at various time points to observe the changes of body temperature, abdominal circumference, stomach and colon pathology before and after administration. Results: Body temperature: The body temperature of each experimental group was increased after modeling (P <0.05). After gavage of Dachengqi Decoction, the body temperature of experimental group and model group decreased (P <0.05). Body temperature was higher in the noon group than that in the Chenshi group and the Baishi group (P <0.05). The body temperature of the non-Shi group, Shen group and Shi-shi group were higher than that of the Chenshi group (P <0.05). (2) Abdominal circumference: Compared with the control group, the abdominal circumference of each experimental group was reduced after modeling (P <0.05). After Dachengqi decoction was given into the stomach, the abdominal circumference of each experimental group was reduced, and the contraction of the experimental group was the most obvious (P < 0.05). Morphological differences were observed at different time points after injection. The most obvious morphological changes were observed at 8:00 in Chenzhou (at 8:00), with the smallest changes at 12:00 (12:00), and the other groups were between 2 groups. CONCLUSION: The morphological changes of administration at different time points are different. Among the 6 hours of daytime, the most obvious changes are taken at the time of Chen, the least obvious changes at noon, and the rest of the hour is between the above two.