【摘 要】
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To identify the inheritance patt and perform fine mapping of ty-5 gene,P1,P2,F1,BC1 and F2 generations were obtained through a cross between CLN32120a-23 (conta
【机 构】
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College of Horticulture, Northeast Agricultural University, Harbin 150030, China
论文部分内容阅读
To identify the inheritance patt and perform fine mapping of ty-5 gene,P1,P2,F1,BC1 and F2 generations were obtained through a cross between CLN32120a-23 (containing ty-5 gene,P1) and S.lycopersicum Moneymaker (fully susceptible,P2).The results showed that resistance of ty-5 gene was determined by a recessive effect.Meanwhile,it was presumed that another resistance gene might be involved in mediating the resistance to tomato yellow leaf curl virus (TYLCV).In this study,fine mapping was used to map TYLCV resistance locus to an interval between NAC1 and TES2461 on the short arm of chromosome 4 with genetic distances of 0.5 and 0.8 cM,respectively.qRT-PCR results showed that four candidate genes,SlNAC1;LOC104229164;LOC101260925 and LOC101261508 having resistance-related expression patts,were the likely target genes of ty-5.In addition,it was found that the codominant marker TES2461 could be used in marker-assisted selection (MAS) breeding.The findings of this research provided the basis for future cloning of ty-5 gene as well as MAS breeding and plant resistance mechanism studies.
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