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In pH 4.4 BR buffer medium, erythrosine (ET) and Chlorphenamine Maleate (CPM) could form ion-association complex, which led to the quenching of fluorescence and synchronous fluorescence, and the significant enhancement of resonance Rayleigh scattering(RRS) of erythrosine. Furthermore, a new RRS spectrum would appear, and the maximum RRS wavelength was located at about 578 nm. The quenched fluorescence and enhanced RRS intensity was directly proportional to the concentration of CPM in the ranges of 0.24―8.0 μg/mL, and 0.008―3.6 μg/mL, respectively. The method has been ap- plied to determine CPM in urine samples with satisfactory results. The mechanisms of the RRS en- hancement and fluorescence quenching were discussed as well.
In pH 4.4 BR buffer medium, erythrosine (ET) and Chlorphenamine Maleate (CPM) could form ion-association complex, which led to the quenching of fluorescence and synchronous fluorescence, and the significant enhancement of resonance Rayleigh scattering (RRS) of erythrosine. , a new RRS spectrum would appear, and the maximum RRS wavelength was located at about 578 nm. The quenched fluorescence and enhanced RRS intensity was directly proportional to the concentration of CPM in the ranges of 0.24-8.0 μg / mL, and 0.008-3.6 μg / mL, respectively. The method has been ap- plied to determine CPM in urine samples with satisfactory results. The mechanisms of the RRS en- hancement and fluorescence quenching were discussed as well.