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目的探讨二甲基甲酰胺(dimethylformamide,DMF)对小鼠心肌细胞(H9c2)的氧化损伤作用及维生素C(vitamin C,VitC)的保护效应。方法使用不同剂量梯度DMF作用心肌细胞24 h、48 h、72 h,同时分别用含有DMF(100 mM)和0.025 mM、0.050 mM、0.100 mM、0.250 mM的VitC培养液培养心肌细胞24 h、48 h、72 h,采用试剂盒测定细胞内超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)的含量以观察细胞氧化应激状态和VitC的保护效应。结果不同浓度的DMF分别作用于心肌细胞24 h、48 h和72 h后使SOD和GSH含量降低,MDA含量升高,产生了明显脂质过氧化并呈现一定的剂量-时间效应关系。保护组VitC在低浓度(0.025 mM、0.050 mM、0.100 mM)时对DMF对心肌细胞的氧化损伤具有保护作用,使SOD和GSH含量升高,MDA降低。保护组VitC在高浓度(0.250 mM)时反而加重DMF对心肌细胞的氧化损伤。结论二甲基甲酰胺(DMF)对小鼠心肌细胞细胞(H9c2)产生了明显的氧化损伤,从而证实DMF所致的氧化应激是其引起细胞毒性的重要机制。小剂量VitC对DMF致心肌细胞的氧化损伤具有明显的保护作用。
Objective To investigate the oxidative damage of dimethylformamide (DMF) to mouse cardiomyocytes (H9c2) and the protective effect of Vitamin C (Vit C). Methods Cardiomyocytes were cultured in different concentrations of DMF for 24 h, 48 h and 72 h respectively. Cardiomyocytes were cultured with VitC medium containing DMF (100 mM) and 0.025 mM, 0.050 mM, 0.100 mM and 0.250 mM for 24 h, 48 h h and 72 h respectively, the contents of superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) in the cells were measured by kit to observe the cellular oxidative stress And VitC protective effect. Results After treated with different concentrations of DMF for 24 h, 48 h and 72 h, respectively, the contents of SOD and GSH decreased and the content of MDA increased, leading to significant lipid peroxidation and some dose-time effects. Protective group VitC protects cardiomyocytes against oxidative damage at low concentrations (0.025 mM, 0.050 mM, 0.100 mM), increases SOD and GSH levels, and decreases MDA. Protection group VitC increased oxidative damage of cardiomyocytes to DMF at high concentrations (0.250 mM). Conclusion Dimethylformamide (DMF) has obvious oxidative damage on mouse cardiomyocytes (H9c2), which proves that the oxidative stress induced by DMF is an important mechanism of its cytotoxicity. Small doses of VitC on DMF induced oxidative damage of myocardial cells has a significant protective effect.