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目前,浙江省兰虫病防治已全面进入基本消灭后期监测阶段,为了更好地实现卫生部颁布的消灭丝虫病近期目标,满足我省各地开展人群丝虫病血清学监测,我们自1990年底始,开展马来丝虫成虫冰冻切片抗原制备及其应用于IFAT的研究,并与广西省寄生虫病防治研究所提供的抗原片进行对照实验。结果显示,本实验制备的马来丝虫成虫冰冻切片抗原(HF-Ag)与广西省寄研所的马来丝虫成虫冰冻切片抗原(GF-Ag)在应用于IFAT中,血清阳性率分别为81.5％和83.33％,无统计学差异(P>0.75),血清抗体GMT有显著差异,HF-Ag显示抗体GMT为1:76,而GF-Ag为1:18,两者间有显著差异(P<0.025)。 本实验在使用包被抗原用的载玻片方面略作改
At present, the prevention and treatment of blue-encephalitis in Zhejiang Province has entered the stage of basically eliminating the latter stage of monitoring. In order to better achieve the immediate goal of eradicating filariasis promulgated by the Ministry of Health and to carry out serodiagnosis of filariasis among the people in our province, since the end of 1990 Initially, the preparation of frozen sectioned antigen of Malayian worm and its application in IFAT were studied, and the control experiment was conducted with the antigenic fragment provided by Institute of Parasitic Diseases, Guangxi Province. The results showed that the frozen-section antigen (HF-Ag) of adult Malayan worm and the frozen section antigen of adult Malayan worm (GF-Ag) from the Institute of Seismology of Guangxi Province were used in IFAT. The positive rates of serum were (81.5%) and 83.33% (P> 0.75). There was a significant difference in serum GMT between HF-Ag and GM-CSF with 1:76 for GF-Ag and 1:18 for GF- (P <0.025). This experiment in the use of coated antigen slide slightly modified