摘要目的在对比增强超声中使用内在固有标记的回顾性门控技术,并与非门控、人工门控以及外部门控的对比增强超声比较有效性。方法5例健康小鼠和5例NEMO敲除的肝脏发育不良小鼠行肝脏对比增强超声成像。记录健康小鼠非特异性微泡的首过效应以及敲除小鼠特异性VEGFR-2微泡的滞留情况。对于回顾性门控,呼吸运动的标记被画为兴趣区。吸气时,兴趣区的信号强度发生变化,从而作为门控信号作用。为了评估准确性,获取非门控的、外在门控和内在固有标记门控的时间强度曲线。 Abstract Objective To compare retrospective gating techniques with contrast-enhanced ultrasound using intrinsic markers and to compare the effectiveness of ultrasound with non-gating, manual gating and external gating. Methods Five healthy mice and five NEMO knockout mice with liver dysplasia underwent liver contrast enhanced ultrasound imaging. The first pass effect of non-specific microbubbles in healthy mice and the retention of knockout mouse-specific VEGFR-2 microbubbles were recorded. For retrospective gating, markers of respiratory motion are drawn as areas of interest. When inhaling, the signal strength of the region of interest changes, acting as a gated signal. To assess accuracy, time-intensity curves for non-gated, extrinsic gating and intrinsic intrinsic label gating were obtained.