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目的:探讨PTEN和FHIT蛋白在上皮性卵巢肿瘤中的表达及意义。方法:收集该院85例上皮性卵巢肿瘤的妇科手术石蜡标本(51例卵巢癌、16例卵巢交界性肿瘤、18例卵巢良性肿瘤),另收集同期其他条件类似的10例正常卵巢组织作对照,采用免疫组织化学SP法检测组织中FHIT和PTEN表达。结果:FHIT在10例正常卵巢组织、18例良性上皮性卵巢肿瘤和16例交界性肿瘤中均呈阳性表达,在上皮性卵巢癌中,其阴性表达率(37.3%,19/51)明显高于良性卵巢肿瘤和交界性卵巢肿瘤,其差异均有统计学意义(P<0.05)。FHIT蛋白阴性表达率在伴淋巴结转移组和不伴淋巴结转移组比较差异也有统计学意义(P<0.05);FHIT蛋白的阴性表达率与病理类型及临床分期无关。卵巢交界性肿瘤和卵巢癌组织中PTEN蛋白表达显著低于卵巢良性肿瘤和正常卵巢组织,其差异均有统计学意义(P<0.05);PTEN蛋白表达与卵巢癌组织分化程度呈正相关(P<0.01),与临床病理分期负相关(P<0.05)。结论:PTEN基因和FHIT基因表达降低或缺失在卵巢癌的发生、侵袭和转移中可能起重要作用,PTEN和FHIT联合检测有助于卵巢肿瘤的早期诊断、早期治疗及生物学行为的评估。
Objective: To investigate the expression and significance of PTEN and FHIT protein in epithelial ovarian tumors. Methods: A total of 85 cases of ovarian epithelial ovarian tumors were collected for gynecological operation (51 cases of ovarian cancer, 16 cases of ovarian borderline tumor and 18 cases of ovarian benign tumor). Another 10 cases of normal ovarian tissue with similar conditions were collected as controls The expression of FHIT and PTEN in tissues was detected by immunohistochemical SP method. Results: FHIT was positively expressed in 10 cases of normal ovarian tissue, 18 cases of benign epithelial ovarian tumor and 16 cases of borderline tumor. The positive expression rate of FHIT in epithelial ovarian cancer (37.3%, 19/51) was significantly higher In benign ovarian tumors and borderline ovarian tumors, the differences were statistically significant (P <0.05). The negative expression rate of FHIT protein in lymph node metastasis group and non-lymph node metastasis group was also significantly different (P <0.05). The negative expression rate of FHIT protein had no correlation with pathological type and clinical stage. The expression of PTEN protein in borderline ovarian tumors and ovarian cancers was significantly lower than that in benign ovarian tumors and normal ovarian tissues (P <0.05). The positive expression of PTEN protein was positively correlated with the differentiation of ovarian cancer (P < 0.01), which was negatively correlated with clinical stage (P <0.05). Conclusion: The decrease or loss of PTEN gene and FHIT gene expression may play an important role in the genesis, invasion and metastasis of ovarian cancer. Combined detection of PTEN and FHIT may be helpful for the early diagnosis, early treatment and biological behavior evaluation of ovarian tumors.