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目的:探讨乳腺癌患者癌组织中CD34和PCNA的表达及临床意义。方法:选择住院乳腺癌手术患者50例、乳腺纤维腺瘤患者40例分为乳腺癌癌旁正常乳腺组织组、乳腺癌组和乳腺纤维腺瘤组。采用酶联免疫法和实时荧光定量PCR法检测CD34、PCNA在3组中的表达。结果:与乳腺癌癌旁正常乳腺组织组相比较,CD34、PCNA蛋白和mRNA水平在乳腺纤维腺瘤组中的表达均略升高,差异无统计学意义(P>0.05),在乳腺癌组中的表达均明显升高,差异有统计学意义(P<0.01);并且乳腺癌组较乳腺纤维腺瘤组升高显著,差异有统计学意义(P<0.01)。结论:CD34、PCNA蛋白和mRNA表达水平的上调说明其在乳腺癌的发生、发展中具有重要作用,并为乳腺癌的诊断和预后提供依据。
Objective: To investigate the expression of CD34 and PCNA in breast cancer and its clinical significance. Methods: 50 cases of hospitalized breast cancer surgery and 40 cases of breast fibroadenoma were divided into normal breast tissue adjacent to breast cancer, breast cancer and fibroadenoma group. Enzyme-linked immunosorbent assay and real-time fluorescence quantitative PCR were used to detect the expression of CD34 and PCNA in the three groups. Results: Compared with normal breast tissue adjacent to breast cancer, the expression of CD34, PCNA protein and mRNA in breast fibroadenoma group were slightly increased, the difference was not statistically significant (P> 0.05), in breast cancer group (P <0.01), and the breast cancer group than the breast fibroadenoma group increased significantly, the difference was statistically significant (P <0.01). Conclusion: The up-regulation of CD34 and PCNA protein and mRNA expression indicates that they play an important role in the occurrence and development of breast cancer and provide the basis for the diagnosis and prognosis of breast cancer.