京尼平是一种环烯醚萜类天然生物交联剂,具有降血糖、调血脂、保肝利胆等生物活性。天然存在的京尼平较少,且质量分数约为0.005%~0.01%。采用β-甘露聚糖酶催化水解栀子苷制备京尼平的工艺路线,通过单因素实验、正交实验确定了β-甘露聚糖酶水解栀子苷的最佳工艺条件:p H为4.5,温度为50℃,底物与酶质量比例1∶2,时间为1 h,产率高达84.49%,纯度达到80%以上。该方法安全、快速、可靠,是一种生产京尼平的新途径。 Genipin is a natural iridoid bio-crosslinker, with hypoglycemic, lipid-lowering, liver and gallbladder and other biological activity. Naturally occurring genipin less, and the mass fraction of about 0.005% ~ 0.01%. The process of preparing genipin by β-mannanase catalyzed hydrolysis of geniposide was studied. The optimum technological conditions for the hydrolysis of geniposide by β-mannanase were determined by single factor experiments and orthogonal experiments: p H was 4.5 , The temperature was 50 ℃, the mass ratio of substrate to enzyme was 1: 2, the time was 1 h, the yield was as high as 84.49% and the purity was over 80%. The method is safe, fast and reliable and is a new way of producing genipin.