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目的:研究Src家族激酶(SFKs)在5’-三磷酸腺苷(ATP)诱导的脊髓背角长时程增强(LTP)中的作用。方法:雄性SD大鼠(250-280 g)。在体电生理记录脊髓腰膨大部背角浅层神经元C-纤维诱发电位,West-ern blotting和免疫组织化学观察脊髓背角SFKs的磷酸化水平和表达部位。结果:ATP应用后30 min和60 min,磷酸化的Src-家族激酶(p-SFKs)的水平明显升高;p-SFKs只表达于小胶质细胞中,星型胶质细胞和神经元中没有表达。脊髓表面应用SFKs抑制剂阻断ATP诱导的LTP。结论:小胶质细胞SFKs可能在ATP诱导的脊髓背角C-纤维诱发电位LTP中发挥重要作用。
AIM: To investigate the role of Src family kinases (SFKs) in the long-term potentiation (LTP) of spinal dorsal horn induced by adenosine 5’-triphosphate (ATP). Methods: Male SD rats (250-280 g). Western blotting and immunohistochemistry were used to observe the phosphorylation and expression of SFKs in spinal dorsal horn. Results: The levels of phosphorylated Src-family kinases (p-SFKs) were significantly increased 30 min and 60 min after ATP administration. P-SFKs were only expressed in microglia, astrocytes and neurons No expression. Spinal surface application of SFKs inhibitors block ATP-induced LTP. Conclusion: Microglial SFKs may play an important role in the ATP-induced LTP of C-fiber evoked potentials in spinal dorsal horn.