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肉桂醇脱氢酶(CAD)是木质素生物合成过程中的一个关键酶。本研究分别提取了“砀山酥梨”及“幸水”梨果肉总RNA,并通过RT-PCR、克隆和测序,成功获得了2个CAD的大小为689bp的cDNA片段,并分别命名为PB-CAD(登录号:FJ478151)和PP-CAD(登录号:FJ478152)。在核苷酸水平上,这两个基因片段只有4个碱基的差异,编码相似性达99.6%的两个氨基酸序列,该序列由229个残基组成。通过蛋白质序列比较,发现梨果实CAD与许多植物中CAD氨基酸序列极为相似,与苹果Malus domestica(AAC06319)、梅Prunus mume(BAE48658)、枇杷Eriobotryajaponica(ABV44810)和葡萄Vitis vinifera(CAO21890)的相似性分别达到97.8%、95%、92.6%和83.4%。
Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in lignin biosynthesis. In this study, total RNA was extracted from “Dasan pear” and “Yushui ” pear pulp, respectively. Two cDNA fragments of 689 bp in size were successfully obtained by RT-PCR, cloning and sequencing. Named PB-CAD (Accession Number: FJ478151) and PP-CAD (Accession Number: FJ478152). At the nucleotide level, the two gene fragments differ by only 4 bases, encoding two amino acid sequences with 99.6% similarity, which consists of 229 residues. Through the comparison of protein sequences, it was found that the CAD of pear fruit is very similar to the CAD amino acid sequence in many plants. The similarity between the CAD of pear fruit and the CAD of Malus domestica (AAC06319), Prunus mume (BAE48658), Eriobotrya japonica (ABV44810) and Vitis vinifera (CAO21890) Reaching 97.8%, 95%, 92.6% and 83.4%.