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目的:探讨乙醇对原代培养颈上神经节神经元(SCGs)细胞质钙稳态的影响。方法:分散、培养新生12 h内大鼠SCGs,应用激光共聚焦显微技术,观察不同浓度乙醇对SCGs细胞质Ca2+浓度([Ca2+]i)的作用。结果:KCl及钙离子载体A23187所诱发的[Ca2+]i增高,可被乙醇(100 mmol/L)显著抑制,但是乙醇(30,100,600 mmol/L)自身却可浓度依赖性增加[Ca2+]i,并且使用无Ca2+外液和氯化镉对该增加作用无影响。结论:乙醇可抑制细胞外Ca2+内流,同时增加细胞质[Ca2+]i,来源可能是胞内钙库的释放。
Objective: To investigate the effects of ethanol on the cytoplasmic calcium homeostasis of primary cultured ganglion neurons (SCGs). Methods: The SCGs were isolated and cultured within 12 h. The effects of different concentrations of ethanol on the cytosolic Ca2 + concentration ([Ca2 +] i) in SCGs were observed by laser scanning confocal microscopy. Results: [Ca2 +] i induced by KCl and calcium ionophore A23187 increased significantly and was inhibited by ethanol (100 mmol / L), but ethanol (30,100,600 mmol / L) increased concentration of [Ca2 +] i by itself, No Ca2 + external use and cadmium chloride had no effect on this increase. Conclusion: Ethanol can inhibit the influx of extracellular Ca2 + and increase the cytoplasmic [Ca2 +] i, which may be due to the release of intracellular calcium.