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根据目前一般书籍记载,关于巧克力琼脂培养基的制作方法,虽然各有异同,但其主要操作方法概括如下:将 pH.7.4—7.6普通琼脂培养基加热至90—95℃,再加入无菌的脱机维羊血或兔血,约为培养基全量的5—10%。摇匀混合后,使冷至50—60℃,分装于灭菌培碟中,或装于中试管中制成斜面培养基,即可供用。按上述方法在具体制作过程中,我们认为有以下几种缺点:1.在每次收到检材后,都需要制做一次新的
According to the current general books, the methods for making chocolate agar medium are similar to each other, but the main methods of operation are summarized as follows: Heat the pH agar medium of pH 7.4-7.6 to 90-95°C, and add sterile Off-line maintenance of blood or rabbit blood, about 5-10% of the total amount of medium. Shake well and mix. Cool to 50-60°C. Dispense in sterile dish or in slant medium in test tube. According to the above method in the specific production process, we believe that there are the following disadvantages: 1. After each sample is received, it is necessary to make a new one.