目的优选金银花多糖除蛋白工艺。方法以多糖损失率和蛋白脱除率为评价指标,选用了3种方法:Sevag法、酶法和酶-Sevag法对金银花多糖提取物进行脱蛋白处理。结果Sevag法、酶法、酶-Sevag法的蛋白脱除率分别为85.72%、73.28%、84.50%,多糖损失率分别为56.57%、31.22%、29.35%。结论酶-Sevag法是金银花多糖除蛋白有效、可行方法。 Objective To optimize the honeysuckle polysaccharide deproteinization process. Methods Polysaccharide loss rate and protein removal rate as the evaluation index, three kinds of methods were selected: Sevag method, enzyme method and enzyme-Sevag method to deproteinize the extract of Lonicera japonica. Results The protein removal rates of Sevag method, enzyme method and enzyme-Sevag method were 85.72%, 73.28% and 84.50%, respectively, and the polysaccharide loss rates were 56.57%, 31.22% and 29.35%, respectively. Conclusions The enzyme-Sevag method is an effective and feasible method for removing honeysuckle polysaccharide.