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利用细胞固定化制备苏云金芽胞杆菌LLP29-M19漂浮剂,为提高其漂浮效果,应用数学统计方法对该菌株漂浮剂配方进行优化.通过单因素试验确定海藻酸钠质量分数为1.0%,CaCl2质量分数为2.0%,空心玻璃微珠质量分数为3.5%;采用Plackett-Burman设计对影响该漂浮剂漂浮效果的8个因素进行筛选,确定漂浮剂配方中的3个重要影响因子为CaCl2、固定化时间和黑色素;通过最陡爬坡试验逼近以上3个因子的最大响应区域后,采用响应面分析获得这3个重要因子的最佳条件,确定LLP29-M19漂浮剂最佳配方:海藻酸钠0.80%、CaCl24.90%、空心玻璃微珠3.2%、菌粉3.0%、黑色素0.012%、苯甲酸钠0.3%、小白鼠饲料0.5%,固定化时间4.74 h.优化后的漂浮剂漂浮效果为1698.25,与响应面数学模型预测值的误差仅1.8%,比初始漂浮剂配方漂浮效果(64.5)提高了25.32倍.
Bacillus thuringiensis LLP29-M19 floating agent was prepared by cell immobilization, in order to improve its floating effect, the formula of floating agent was optimized by mathematical statistics method.The single factor test was used to determine the mass fraction of sodium alginate 1.0%, CaCl2 mass fraction Was 2.0%, and the content of hollow glass microbeads was 3.5%. The Plackett-Burman design was used to screen the eight factors influencing the floating effect of the floating agent. The three important influencing factors in the formulation were determined as CaCl2, the immobilization time And melanin. After the steepest ascent test approached the maximum response region of the above three factors, the optimal conditions of these three important factors were obtained by response surface analysis to determine the optimal formula of LLP29-M19 floater: sodium alginate 0.80% , 24.90% of CaCl 2, 3.2% of hollow glass beads, 3.0% of bacterial powder, 0.012% of melanin, 0.3% of sodium benzoate, 0.5% of mouse feed and 4.74 hours of immobilization. The floating effect of the optimized floating agent is 1698.25, Response surface mathematical model prediction error of only 1.8%, than the initial floating agent formulation floating effect (64.5) increased 25.32 times.