The paper described experimental investigation of heat transfer and single-phase pressure drop through tubes with different rotor-assembled strands inserted in the Reynolds number range of 800-9000 with lubricant as working fluid. In the experiment, fixed mounts were employed to eliminate the entrance effect. The experimental results showed that the employment of fixed mounts led to a visible bias of friction factor in the laminar regime while it could not affect the Nusselt numbers significantly. Experiment for the tube inserted with rotors-assembled strand showed remarkable improvement for heat transfer with the Nusselt number increased by 200%-225% in the laminar regime and 125%-160% in the transitional regime. Meanwhile, the friction factor increased inevitably by 200%-300% within the same range of Reynolds number. The comparison of different rotor-assembled strands in-serted tubes and plain tube showed that the heat transfer benefited from the increase of the diameter of rotor-assembled strand with the same lead and the decrease of the lead of rotor-assembled strand, so does the friction factor. Based on experimental data and thorough multivariant linear normal regression method, the correlations of average Nusselt number and friction factor are established. The paper said experimental investigation of heat transfer and single-phase pressure drop through tubes with different rotor-assembled strands inserted in the Reynolds number range of 800-9000 with lubricant as working fluid. In the experiment, fixed mounts were employed to eliminate the entrance effect. The experimental results showed that the employment of fixed mounts led to a visible bias of the friction factor in the laminar regime while it could not affect the Nusselt numbers significantly. Experiment for the tube inserted with rotors-assembled strand showed remarkable improvement for heat transfer with the Nusselt number increased by 200% -225% in the laminar regime and 125% -160% in the transitional regime. Meanwhile, the friction factor increased inevitably by 200% -300% within the same range of Reynolds number. The comparison of different rotor-assembled strands in-serted tubes and plain tube showed that the heat transfer benefited from the increase of the diameter of rotor-ass embled strand with the same lead and the decrease of the lead of rotor-assembled strand, so does the friction factor. Based on experimental data and thorough multivariant linear normal regression method, the correlations of average Nusselt number and friction factor are established.