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目的 :探讨在儿茶酚胺诱导的心肌损伤过程中 ,心肌细胞的凋亡相关基因 Fas和 Bcl 2的蛋白表达改变及葛根素干预的影响。方法 :5 0只健康雄性 SD大鼠随机分成 3组 :心肌损伤组 (2 0只 )、葛根素干预组 (2 0只 )和对照组 (10只 )。动物模型仿用异丙肾上腺素 (ISO)皮下注射致心肌损伤方法。心肌组织切片分别行 HE及 Fas和 Bcl 2蛋白表达的免疫组织化学染色检测。结果 :心肌损伤组 :Fas蛋白和 Bcl 2蛋白的表达等级均上调 (P均 <0 .0 0 1) ,Fas蛋白的表达水平更高 (P<0 .0 0 1)。葛根素干预组 :心肌组织的 HE病理损害等级明显减轻 (P<0 .0 5 ) ;Fas蛋白表达等级下调 (P<0 .0 5 )。结论 :在儿茶酚胺诱导的心肌损伤过程中 ,Fas和 Bcl 2的蛋白表达水平表现出高水平的差异 ,葛根素能抑制损伤过程中 Fas蛋白的表达。
OBJECTIVE: To investigate the changes of protein expression of Fas and Bcl 2 in cardiomyocytes during catecholamine-induced myocardial injury and the effect of puerarin intervention. Methods: Fifty healthy male Sprague-Dawley rats were randomly divided into 3 groups: myocardial injury group (20 rats), puerarin intervention group (20 rats) and control group (10 rats). Animal model imitate isoproterenol (ISO) caused by subcutaneous injection of myocardial injury method. Myocardial tissue sections were detected by HE and Fas and Bcl 2 protein expression by immunohistochemical staining. Results: In myocardial injury group, the expression of Fas protein and Bcl-2 protein were up-regulated (P <0.01), and the expression of Fas protein was higher (P <0.01). Puerarin intervention group: The level of HE pathological lesion in myocardial tissue was significantly reduced (P <0.05); the expression of Fas protein was down-regulated (P <0.05). CONCLUSION: In the catecholamine-induced myocardial injury, Fas and Bcl 2 protein expression levels showed a high level of difference, puerarin can inhibit the expression of Fas protein during injury.