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目的幽门螺杆菌（Ｈｐ）与活动性胃炎、消化性溃疡关系密切。而ＣａｇＡ基因被认为是幽门螺杆菌的毒力标志。探讨ＣａｇＡ基因型幽门螺杆菌和细胞因子——肿瘤坏死因子（ＴＮＦ－α）、白细胞介素（ＩＬ－６、ＩＬ－８）在上消化道疾病中的作用。方法（１）用聚合酶链反应（ＰＣＲ）技术，对１１８例胃十二指肠患者的胃粘膜进行ＣａｇＡ基因检测。（２）用酶联免疫吸附法（ＥＬＩＳＡ）测定胃粘膜培养上清中ＴＮＦ－α、ＩＬ－６、ＩＬ－８的含量。结果６７例Ｈｐ感染患者中５０例检出ＣａｇＡ基因。其中１５例十二指肠溃疡患者全部检出ＣａｇＡ基因，１０例胃溃疡患者中８例检出ＣａｇＡ基因，４２例慢性胃炎中２７例检出ＣａｇＡ基因。Ｈｐ阳性组的胃粘膜培养上清中ＴＮＦ－α、ＩＬ－８、ＩＬ－６的含量显著高于Ｈｐ阴性组（Ｐ＜０．０１），ＣａｇＡ＋Ｈｐ组胃粘膜培养上清中ＩＬ－８的浓度显著高于ＣａｇＡＨｐ组（Ｐ＜０．０１）。ＴＮＦ－α与ＩＬ－８、ＩＬ－６呈正相关。ＴＮＦ－α、ＩＬ－８、ＩＬ－６的浓度在有Ｈｐ感染的消化性溃疡和慢性胃炎之间差异无显著意义（Ｐ＞０．０５）。组织学表明，ＣａｇＡ＋Ｈｐ与胃粘膜的中性粒细胞浸润程度有关，而与肠化生、腺体萎缩，不典型增生的程度无?
Purpose Helicobacter pylori (Hp) and active gastritis, peptic ulcer are closely related. The CagA gene is considered to be a virulence marker of Helicobacter pylori. To investigate the role of CagA genotype Helicobacter pylori and cytokines - tumor necrosis factor (TNF-α), interleukin (IL-6, IL-8) in upper gastrointestinal diseases. Methods (1) CagA gene was detected in gastric mucosa of 118 patients with gastroduodenal by Polymerase Chain Reaction (PCR). (2) The contents of TNF-α, IL-6 and IL-8 in gastric mucosa culture supernatant were determined by enzyme-linked immunosorbent assay (ELISA). Results CagA gene was detected in 50 out of 67 cases of Hp infection. CagA gene was detected in 15 cases of duodenal ulcer, CagA gene in 8 cases of gastric ulcer in 10 cases and CagA gene in 27 cases of 42 cases of chronic gastritis. The content of TNF-α, IL-8 and IL-6 in gastric mucosa culture supernatant of Hp positive group was significantly higher than that in Hp negative group (P <0.01). The concentration of IL-8 in supernatant of gastric mucosa in CagA + Hp group Significantly higher than CagAHp group (P <0.01). TNF-α and IL-8, IL-6 was positively correlated. There were no significant differences in the concentrations of TNF-α, IL-8 and IL-6 between peptic ulcer and chronic gastritis with Hp infection (P> 0.05). Histology showed that CagA + Hp and gastric mucosal neutrophil infiltration, and intestinal metaplasia, gland atrophy, atypical hyperplasia level?