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目的探寻后肢去负荷过程中,能够阻止软骨退化的对抗措施。方法 SD大鼠分3组(每组8只):对照组(Con)、尾吊组(TS)、尾吊+被动运动组(TSP)。尾吊21天期间,TSP组接受由训练装置提供的被动运动训练。利用μCT与组织切片检测大鼠股骨远端软骨的变化。结果μCT结果表明,TS与TSP组大鼠股骨远端的关节软骨厚度与Con比明显下降;TS的软骨体积较Con与TSP显著下降,而Con与TSP间没有显著差异;TS与TSP的软骨的衰减系数较Con明显升高。组织切片表明,TS与TSP的软骨厚度较Con明显下降,番红O—固绿染色显示TS与TSP的基质染色程度较Con下降,软骨细胞出现减少的现象。此外,μCT所测厚度与组织切片所测厚度高度相关。结论大鼠尾吊会导致关节软骨退化,然而被动运动不能有效的阻止尾吊引起的软骨退化。
Objective To explore the countermeasures to prevent the degeneration of cartilage in the process of the hindlimb deloading. Methods SD rats were divided into 3 groups (8 in each group): control group (Con), tail suspension group (TS), tail suspension + passive exercise group (TSP). During the 21-day tail suspension, the TSP group received passive exercise training provided by the training device. Changes of rat distal femur cartilage detected by μCT and tissue sections. Results The results of μCT showed that the thickness of the articular cartilage and Con ratio in the distal femur of TS and TSP groups decreased significantly. The cartilage volume of TS decreased significantly compared with Con and TSP, but there was no significant difference between Con and TSP. The attenuation coefficient was significantly higher than that of Con. Tissue sections showed that the cartilage thickness of TS and TSP decreased significantly compared with that of Con. Safranin O-solid green staining showed that the stromal staining of TS and TSP was decreased compared with that of Con and chondrocytes were decreased. In addition, the thickness measured by μCT is highly correlated with the thickness measured by histological sections. Conclusion The rat tail suspension can cause the degeneration of articular cartilage. However, passive exercise can not effectively prevent cartilage degeneration caused by tail suspension.