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目的:建立以高效液相色谱法同时测定黄连羊肝丸中黄芩苷和盐酸小檗碱含量的方法。方法:采用Chromatrex C182(2605 0n mmm,×柱4.6温m为m25,5℃μm,进)色样谱量柱为,20流μ动L相。为结甲果醇:-黄1%芩三苷乙和胺盐(酸加小入檗磷碱酸检调p测H浓值度至3分.0别,3在8∶166.20)～,8流0.0速μ为g1..0m Lm-L1.(rmi=n0-.199,9检9测)波、1.长1为～9.9μg.mL-1(r=0.999 9)范围内线性关系良好;平均加样回收率(n=9)分别为100.16%和100.86%,RSD分别为0.31%、1.86%。结论:该方法简便、准确、重复性好,可用于同时测定黄连羊肝丸中黄芩苷和盐酸小檗碱的含量。
Objective: To establish a method for simultaneous determination of baicalin and berberine hydrochloride in Huanglian Yanggan Pill by high performance liquid chromatography. Methods: Chromatrex C182 (2605 nm, × 4.6 column temperature m m25,5 ℃ μm, into) the color of the chromatographic column, 20 flow μ moving L phase. For fructose fructose: - yellow 1% Scutellagen glycosides B and amine salt (acid plus small berberine phosphoric acid detection p measured H concentration to 3 points .0 do not, 3 at 8:166.20) ~, 8 Flow rate 0.0 μ is g1..0m Lm-L1. (Rmi = n0-.199,9 test 9 test) wave, 1. Long 1 is ~ 9.9μg.mL-1 (r = 0.999 9) within the linear relationship The average recoveries (n = 9) were 100.16% and 100.86% respectively, with RSDs of 0.31% and 1.86%, respectively. Conclusion: The method is simple, accurate and reproducible. It can be used for simultaneous determination of baicalin and berberine in Huang Lian Yang Gan Wan.