Objective:To determine the prevalence of viral haemoparasites in prospective Nigerian blood donors.Methods:Ethical clearance was obtained and informed consent questionnaires were distributed to blood donors to obtain their demographical data.A total of 186 blood donors from LAUTECH Teaching Hospital,Osogbo were tested for hepatitis A virus(HAV),hepatitis B virus(HBV) and hepatitis C virus(HCV) using rapid test kit and Enzyme Linked Immunosorbent Assay.Results:The highest prevalence of blood transmitted infections was 182(97.85%) while the prevalence of HIV,HAV,HBV and HCV were 6.45%,97.85%,14.52% and 3.23% respectively.Highest seroprevalence for hepatitis A,B and C occurred among low risk occupation.There was no significant association between all the hepatitis viruses and demographic factors except occupation with P value of 0.002 7.Hepatitis A,B and C seropositive blood donors on average tend to have PCV within the normal reference range.Out of the 27 hepatitis B positive blood donors,22 were donating blood for the first time while 5 were repeat donors.None of the hepatitis C seropositive donors have been exposed to blood or any form of its products and were all donating blood for the first time.However,the distribution of donor type for HAV is random.Conclusions:The presence of HAV,HBV,HCV and HIV among prospective donors in Nigeria particularly the alarming prevalence of HAV.These infections can be transmitted to recipients if proper screening is not carried out,hence they should be included as a routine test for blood donors. Objective: To determine the prevalence of viral haemoparasites in prospective Nigerian blood donors. Methods: Ethical clearance was obtained and accorded consent questionnaires were distributed to blood donors to obtain their demographical data. A total of 186 blood donors from LAUTECH Teaching Hospital, Osogbo were tested for hepatitis A virus (HAV), hepatitis B virus (HBV) and hepatitis C virus (HCV) using rapid test kit and Enzyme Linked Immunosorbent Assay. Results: The highest prevalence of blood transmitted infections was 182 (97.85%) while the prevalence of HIV, HAV, HBV and HCV were 6.45%, 97.85%, 14.52% and 3.23% respectively. Highest seroprevalence for hepatitis A, B and C occurred among low risk occupation. There was no significant association between all the hepatitis viruses and demographic factors except occupation with P value of 0.002 7. Hepatitis A, B and C seropositive blood donors on average tend to have PCV within the normal reference range. Out of the 27 hepatitis B positive blood donor s, 22 were donating blood for the first time while 5 were repeat donors. None of the hepatitis C seropositive donors have been exposed to blood or any form of its products and were all donating blood for the first time. However, the distribution of donor type for HAV is random.Conclusions: The presence of HAV, HBV, HCV and HIV among prospective donors in Nigeria particularly the alarming prevalence of HAV. These infections can be transmitted to recipients if proper screening is not carried out, subsequently they should be included as a routine test for blood donors