论文部分内容阅读
目的 探讨结肠癌患者N末端截短型p63基因(ΔNp63)和核转录因子κB(NF-κB)的表达及意义.方法 采用免疫组织化学法检测结肠癌患者ΔNp63和NF-κB的表达;采用实时荧光定量聚合酶链反应(qRT-PCR)和Western blot检测ΔNp63和NF-κB信使核糖核酸(mRNA)和蛋白的表达;采用半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)活性检测试剂盒检测凋亡蛋白Caspase-3表达.结果 与对照组比较,结肠癌患者ΔNp63蛋白表达升高35.57％,NF-κB蛋白表达升高58.93％,差异有统计学意义(P＜0.05);结肠癌患者ΔNp63和NF-κB mRNA和蛋白表达均升高,差异有统计学意义(P＜0.05);结肠癌患者Caspase-3活性升高,差异有统计学意义(P＜0.05).结论 结肠癌患者ΔNp63和NF-κB呈过度表达状态;ΔNp63和NF-κB的过表达在结肠癌发生、发展机制中可能起到重要作用.“,”Objective To investigate the expression and effect of ΔNp63 and NF-κB in patients with colon cancer.Methods The expressions of ΔNp63 and NF-κB in patients with colon cancer were detected by immunohistochemical method.The mRNA and protein expressed by ANp63 and NF-κB were detected by real-time polymerase chain reaction (RT-PCR) and Western blot.The expression of Caspase-3 in apoptotic cells was detected by Caspase-3 activity assay kit.Results Compared with the control group,the level of protein expressed by ANp63 rose by 35.57％ and the level of protein expressed by NF-κB rose by 58.93％,the difference was statistically significant (P＜ 0.05).The mRNA and protein expressed by ΔNp63 and NF-κB were obviously up-regulated,the differences were statistically significant (P ＜ 0.05).The activity of Caspase-3 in the colon cancer group was obviously increased,the difference was statistically significant (P ＜ 0.05).Conclusions The expressions of ΔNp63 and NF-κB in colon cancer patients are over-expressed,and the over-expressed ANp63 and NF-κB may play important roles in the development and progression of colon cancer.