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目的:研究乌司他丁对重度失血性休克大鼠复苏后血清和脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)和肿瘤坏死因子o(TNF-α)含量的影响。方法:将42只大鼠随机分为假手术组(A),单纯缺血再灌注组(B)和乌司他丁治疗组(C),通过颈动脉放血建立失血性休克模型,B、C组根据休克时间分为30、60、120 min三个亚组,各组大鼠在复苏后测定血清和脑组织中MDA、SOD和TNF-α的含量。结果:大鼠休克30 min复苏血清MDA和TNF-α含量较A组升高,但C组低于B组,血清SOD和脑组织中MDA、SOD和TNF-α含量未出现明显变化;大鼠休克60 min复苏血清MDA、TNF-α含量进一步升高,脑组织TNF-α含量开始升高,但C组低于B组;大鼠休克120 min复苏,血清和脑组织MDA、TNF-α含量较A组明显升高,C组低于B组,血清和脑组织SOD活力明显下降,但C组高于B组。结论:在重度失血性休克情况下进行复苏,大鼠将出现全脑缺血再灌注损伤,应用乌司他丁可在一定程度上减轻该损伤。
Objective: To investigate the effects of ulinastatin on the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and tumor necrosis factor (TNF) -α in serum and brain tissue after severe hemorrhagic shock in rats. Methods: Forty-two rats were randomly divided into sham operation group (A), ischemia reperfusion group (B) and ulinastatin treatment group (C). Hemorrhagic shock model was established by carotid artery exsanguination. B and C According to the shock time, the rats were divided into three subgroups: 30, 60 and 120 min. The levels of MDA, SOD and TNF-α in serum and brain tissue of rats in each group were measured after resuscitation. Results: Serum levels of MDA and TNF-α in resuscitation 30 min after shock were higher than those in group A, but those in group C were lower than those in group B. Serum SOD and contents of MDA, SOD and TNF-α in brain tissue did not change significantly. Rats The levels of serum TNF and TNF-α in the resuscitation 60 minutes after shock increased further, the content of TNF-α in brain tissue began to increase, but the level in group C was lower than that in group B; Which was significantly higher than that of group A, while that of group C was lower than that of group B. The activity of SOD in serum and brain tissue was significantly decreased, but in group C was higher than that in group B. CONCLUSIONS: In the case of severe hemorrhagic shock resuscitation, there will be global cerebral ischemia-reperfusion injury, the application of ulinastatin can reduce the damage to some extent.