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目的:研究山胡椒Lindera glauca中的化学成分及其抗肿瘤转移活性。方法:综合运用硅胶柱色谱、凝胶柱色谱、HPLC制备色谱等多种色谱法分离纯化山胡椒中的化学成分;采用NMR等波谱学方法鉴定化合物结构,进一步以Tran-swell趋化实验方法测定化合物抗肿瘤转移作用。结果:从山胡椒乙醇提取物中分离得到10个化合物,分别为樟苍碱(1),N-甲基樟苍碱(2),瑞枯灵(3),紫堇碱(4),N-反式阿魏酸酪酰胺(5),N-顺式阿魏酸酪酰胺(6),芒籽香碱(7),降异紫堇定碱(8),[9,9,9-2H3]-(1S*,3S*,4S*,8S*)-p-Menthane-3,8-diol(9),[9,9,9-2H3]-(1S*,3R*,4S*,8S*)-p-Menthane-3,8-diol(10)。抗肿瘤转移实验结果表明化合物1,2,4,5,7和9均有明确的抗肿瘤转移作用,其中化合物1,4和5的抗肿瘤转移活性较强。结论:化合物3为首次从该植物中分离得到,化合物9,10为首次从该属植物中分离得到;化合物1,4和5的抗肿瘤转移活性较强。
Objective: To study the chemical constituents and antitumor activity of Lindera glauca in pepper. Methods: The constituents were isolated and purified by silica gel column chromatography, gel column chromatography and HPLC-preparative chromatography. The structure of compounds was identified by NMR spectroscopy and further by Tran-swell chemotaxis assay Anti-tumor metastatic effect of compounds. Results: Ten compounds were isolated from the ethanolic extract of Morus alba. They were zakuride (1), N-methyl campharine (2), Ruikuling (3), corydaline Trans-ferulic acid amide (5), N-cis-ferulic acid amide (6), mangosteen (7), isovidin (8), [9,9,9- (9), [9,9,9-2H3] - (1S *, 3R *, 4S *, 3S *, 4S *, 8S * 8S *) - p-Menthane-3,8-diol (10). Anti-tumor metastasis results show that compounds 1, 2, 4, 5, 7 and 9 have definite anti-tumor metastasis effects, of which compound 1, 4 and 5 have stronger antitumor activity. CONCLUSION: Compound 3 was isolated from this plant for the first time. Compounds 9 and 10 were isolated from this genus for the first time. Compounds 1, 4 and 5 have stronger antitumor activity.