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目的:通过验证罗勒(全草)提取物(OBL)对肾性高血压模型大鼠的降压作用,探讨OBL的降压作用机理。方法:正常Sprague-Dawley(SD)大鼠适应性喂养1周后测基础血压。然后进行左肾动脉狭窄手术。手术后4周,形成两肾一夹(2K1C)肾性高血压。将高血压大鼠60只随机分成5组:罗勒提取物高剂量组(H,400 mg/kg)、罗勒提取物中剂量组(M,200 mg/kg)、罗勒提取物低剂量组(L,100 mg/kg)、卡托普利组(Cap,30 mg/kg)、高血压模型组(Model)。从肾动脉狭窄手术后4周开始灌胃给予相应药物,持续4周,每周末测血压;外加假手术组(Sham)10只。处死动物取血测血管紧张素Ⅱ(AngⅡ)、内皮素(ET)、超氧化物歧化酶(SOD)、丙二醛(MDA)。结果:两肾一夹(2K1C)术后4周,假手术组血压为(138±2.04)mmHg,模型组血压为(201±3.69)mmHg,两组血压差异有统计学意义(P<0.05)。同时AngⅡ、ET以及MDA含量明显增高;而SOD活性降低。给予罗勒提取物后血压(收缩压和舒张压)均有下降。ET、MDA明显下降、而SOD活性增高(P<0.05),且作用强于卡托普利组(P<0.05)。同时AngⅡ也明显下降。结论:罗勒提取物对肾性高血压大鼠具有降压作用,其降压作用机制可能与通过抗氧化反应来保护内皮细胞,改善血管内皮功能紊乱,抑制缩血管物质(内皮素、血栓素A2、血管紧张素Ⅱ)的释放有关。
Objective: To verify the antihypertensive effect of OBL by verifying the antihypertensive effect of basil extract (OBL) on renal hypertensive rats. METHODS: Basal blood pressure was measured in normal Sprague-Dawley (SD) rats 1 week after adaptive feeding. Then left renal artery stenosis surgery. Four weeks after surgery, two kidneys and one clip (2K1C) of renal hypertension develop. Sixty hypertensive rats were randomly divided into 5 groups: high dose of basil extract (H, 400 mg/kg), dose of basil extract (M, 200 mg/kg), and low dose of basil extract (L , 100 mg/kg), Captopril (Cap, 30 mg/kg), Hypertension Model Group (Model). From the 4th week after surgery of renal artery stenosis, corresponding drugs were given by gavage for 4 weeks, and blood pressure was measured every weekend; 10 sham groups were added. Animals were sacrificed and blood was taken for angiotensin II (Ang II), endothelin (ET), superoxide dismutase (SOD) and malondialdehyde (MDA). RESULTS: Four weeks after operation, the blood pressure in the sham-operated group was (138±2.04) mmHg, and the blood pressure in the model group was (201±3.69) mmHg. The difference in blood pressure between the two groups was statistically significant (P<0.05). . At the same time, the contents of AngII, ET and MDA increased significantly, while the activities of SOD decreased. Blood pressure (systolic blood pressure and diastolic blood pressure) decreased after giving Basil extract. ET and MDA significantly decreased while SOD activity increased (P<0.05), and the effect was stronger than that of captopril group (P<0.05). At the same time, AngII also decreased significantly. Conclusion: Basil extract has antihypertensive effect on renal hypertensive rats, and its antihypertensive mechanism may be related to the protection of endothelial cells by antioxidation, improvement of vascular endothelial dysfunction, and inhibition of vasoconstrictor substances (endothelin, thromboxane A2 Is related to the release of angiotensin II).