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医学科研实验中小鼠采血一直比较困难。现在常用的方法有断颈采血法、挖眼球采血法和眶后静脉丛采血法。前两法为一次性采血或重残采血,后一种方法需要特制的采血器具与较熟练的技术,且小鼠易窒息死亡,每次采血量仅0.5m1。我们试用一种新的尾静脉采血法,此法简便、易行,小鼠不易死亡,可反复采血,每次采血量较多,现介绍于下。一、器材准备 1.真空泵:30L/min 2.吸滤瓶:常用500～1000ml,瓶中放盛血的试管,瓶口盖一块厚橡皮板,其中心有一直径约为1.5cm的圆孔。
It has been difficult for mice to collect blood in medical research experiments. The commonly used methods are neck blood sampling, digging eye ball blood sampling, and retro-orbital venous plexus blood sampling. The first two methods are one-time blood collection or heavy blood collection. The latter method requires special blood collection equipment and more skilled techniques, and the mice are easy to suffocate and die, and the amount of blood collected is only 0.5m1 at a time. We have tried a new method of collecting blood from the tail vein. This method is simple and easy. The mice are not easy to die, blood can be collected repeatedly, and the amount of blood collected each time is higher. First, equipment preparation 1. Vacuum pump: 30L/min 2. Suction filter bottle: commonly used 500 ~ 1000ml, the bottle into the blood test tube, the bottle cover a thick rubber plate, its center has a diameter of about 1.5cm round hole.