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MADS-box蛋白在植物生长发育及抗逆等过程中均发挥重要功能。本实验室根据甘薯近缘野生种I.trifida基因组序列,在甘薯栽培种徐薯22(Ipomoea batatas(L.)Xu22)中克隆到一个STMADS11亚家族MADS-box基因,命名为IbMADS11-Like。实时定量RT-PCR分析表明,IbMADS11-Like基因在甘薯根中大量表达,并且随着块根的形成和膨大表达量逐渐降低,表明该基因可能参与了甘薯块根的发育过程。胁迫处理分析表明,IbMADS11-Like基因的表达受干旱、盐和高温的诱导,而低温则抑制其表达。此外,IbMADS11-Like基因对ABA、IAA、ZT、BR、ACC、JA及GA等激素的处理也有不同程度的响应,暗示IbMADS11-Like基因可能参与了甘薯生长发育及胁迫的调控过程。这些结果为进一步分析IbMADS11-Like基因在甘薯块根发育和胁迫响应中的功能奠定了基础。
MADS-box protein plays an important role in the process of plant growth and resistance. According to the I. trifida genomic sequence of wild sweet potato, we cloned a STMADS11 subfamily MADS-box gene in the sweet potato cultivar Xpomoea batatas (L.) Xu22 and named it as IbMADS11-Like. Real-time quantitative RT-PCR analysis showed that IbMADS11-Like gene was abundantly expressed in sweetpotato roots, and gradually decreased with the formation and expansion of tuberous roots, indicating that the gene may be involved in the development of sweetpotato roots. Stress analysis showed that the expression of IbMADS11-Like gene was induced by drought, salt and high temperature, while its expression was inhibited by low temperature. In addition, the gene IbMADS11-Like also responded differently to ABA, IAA, ZT, BR, ACC, JA and GA hormones, suggesting that the IbMADS11-Like gene may be involved in the regulation of sweet potato growth and stress. These results lay the foundation for further analysis of the function of IbMADS11-Like gene in root development and stress response of sweet potato.