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应用常规方法建立了３株稳定分泌抗金黄色葡萄球菌Ｃ１型肠毒素（ＳＥＣ１）单克隆抗体（ＭｃＡｂ）的小鼠杂交瘤细胞系Ｂ３、Ｃ４和Ｇ８。其中Ｂ３和Ｃ４均为ＩｇＧ１（ｋ），Ｇ８为ＩｇＧ２ａ（ｋ）。Ｂ３和Ｇ８与ＳＥＡ、ＳＥＢ及ＳＥＤ均无交叉反应；Ｃ４虽与ＳＥＡ和ＳＥＤ无交叉反应，但与ＳＥＢ有交叉反应。间接ＥＬＩＳＡ测定小鼠腹水效价为１０－５～１０－８。应用识别不同表位的ＭｃＡｂ建立了双ＭｃＡｂ夹心ＥＬＩＳＡ法检测ＳＥＣ１，敏感性可达１ｎｇ／ｍｌ。
Three mouse hybridoma cell lines B3, C4, and G8 that stably secrete anti-Staphylococcus aureus type 1 (SEC1) monoclonal antibodies (McAbs) were established using routine methods. Among them, B3 and C4 are both IgG1(k), and G8 is IgG2a(k). There was no cross reaction between B3 and G8 and SEA, SEB and SED. Although C4 had no cross reaction with SEA and SED, it had cross reaction with SEB. Indirect ELISA assay mouse ascites titer of 10-5 ~ 10-8. Using a McAb that recognizes different epitopes, a double McAb sandwich ELISA was used to detect SEC1 with a sensitivity of 1 ng/ml.