建立了生鲜牛乳中8种氟喹诺酮类药物的超高效液相色谱串联质谱的测定方法。生鲜牛乳经QuEChERS萃取剂提取,QuEChERS净化剂净化后,经氮气流吹干浓缩后,以超高效液相色谱串联质谱测定,稳定同位素内标法定量。本方法对氟喹诺酮类药物的测定线性范围为2.0~100μg/kg。在2.0、10、100μg/kg低、中、高三个浓度的回收率为80%~120%。批内、批间精密度小于20%。本方法简便、快速、灵敏,适用于生鲜牛乳中氟喹诺酮类药物残留的大批量筛选和定量测定。 A method for the determination of 8 fluoroquinolones in fresh milk by ultra performance liquid chromatography-tandem mass spectrometry was established. Raw milk was extracted by QuEChERS extractant, purified by QuEChERS scavenger, then dried by nitrogen flow and concentrated by high performance liquid chromatography-tandem mass spectrometry, and quantified by stable isotope internal standard method. The method for the determination of fluoroquinolones linear range of 2.0 ~ 100μg / kg. The recoveries of low, medium and high concentrations of 2.0,10,100μg / kg were 80% ~ 120%. Batch, inter-batch precision less than 20%. The method is simple, rapid and sensitive, and is suitable for large-scale screening and quantitative determination of fluoroquinolone residues in fresh milk.