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目的观察金福安汤对人肺腺癌A549细胞增殖、p120ctn磷酸化、细胞黏附及迁移的影响并探讨其治疗肺癌的作用机制。方法培养A549细胞,分为空白对照组,金福安汤低、中、高剂量组,环磷酰胺组,分别给予含空白血清和含药血清培养液干预。检测各组A549细胞OD值并计算抑瘤率;刺激A549细胞p120ctn磷酸化后,检测各组A549细胞p120ctn灰度值;并观察各组p120ctn磷酸化的A549细胞黏附和迁移。结果金福安汤高、中、低剂量组A549细胞OD值与空白对照组比较差异有统计学意义,且呈剂量相关性(P<0.05或P<0.01);金福安汤中剂量组A549细胞p120ctn磷酸化程度较空白对照组显著降低(P<0.05);金福安汤中、高剂量组A549细胞的黏附能力较空白对照组高(P<0.05),金福安汤各剂量组A549细胞的迁移能力较空白对照组下降(P<0.05)。结论金福安汤具有抑制A549细胞增殖及其p120ctn磷酸化、促进细胞黏附和抑制迁移的作用。
Objective To observe the effect of Jinfu An Tang on proliferation, p120ctn phosphorylation, cell adhesion and migration of human lung adenocarcinoma A549 cells and to explore its mechanism of action in lung cancer. Methods A549 cells were cultured and divided into blank control group, Jinfuan decoction low, medium and high dose groups and cyclophosphamide group, respectively. The OD value of A549 cells in each group was detected and the tumor inhibition rate was calculated. After phosphorylation of p120ctn in A549 cells was stimulated, the gray value of p120ctn in A549 cells was detected; and the adhesion and migration of p120ctn phosphorylated A549 cells in each group were observed. Results The OD value of A549 cells in high, medium and low doses of Jinfu’an Decoction was significantly lower than that of the blank control group (P <0.05 or P <0.01). The p120ctn The phosphorylation of A549 cells was significantly lower than that of the blank control group (P <0.05), and the adhesion ability of the middle and high doses of Jinfu’an decoction group was higher than that of the blank control group (P <0.05) Compared with the blank control group decreased (P <0.05). Conclusion Jin Fu An decoction can inhibit the proliferation of A549 cells and its p120ctn phosphorylation, promote cell adhesion and inhibit the migration.