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目的探讨咖啡因对慢性刺激小鼠行为和学习记忆的影响。方法选取28只C57BL/6J小鼠,随机分为正常组(n=10):空白+0.15ml生理盐水;刺激组(n=8):慢性刺激+0.15ml生理盐水;药物组(n=10):慢性刺激+15mg/kg咖啡因。刺激组和药物组分别孤养,并接受持续10周的慢性不可预知温和刺激。结合旷场实验和Morris水迷宫方法,评价小鼠的行为和学习记忆能力。结果 10周后刺激组和药物组的水平穿越格子数[(118.50±12.20)次,(80.10±16.02)次)]都明显高于正常组(49.00±10.05)次(P<0.01);水迷宫测试中,药物组定位航行训练第1天的潜伏期明显短于正常组和刺激组[(13.12±2.27)s,(27.34±5.00)s,(30.62±4.54)s](P<0.05);药物组和正常组体重增加量[(3.40±0.37)g,(5.40±0.69)g]有明显差异(P<0.05),10周后药物组和正常组体重[(24.60±0.43)g,(26.60±0.62)g]有明显差异(P<0.05)。结论长期慢性低剂量咖啡因注射可以减缓慢性刺激下小鼠体重增加,同时还能加强刺激状态下小鼠的空间学习记忆能力;长期慢性刺激也能加强小鼠的探索行为。
Objective To investigate the effects of caffeine on behavior and learning and memory of chronic-challenged mice. Methods 28 C57BL / 6J mice were randomly divided into normal group (n = 10): blank + 0.15ml saline; stimulation group (n = 8): chronic stimulation + 0.15ml saline; ): Chronic irritation + 15 mg / kg caffeine. The stimulation group and the drug group were isolated and maintained under chronic unpredictable mild stimulation for 10 weeks. In combination with open-field experiments and the Morris water maze method, mice were evaluated for behavioral and learning and memory abilities. Results After 10 weeks, the number of crossing the squares of the stimulation group and the drug group was significantly (118.50 ± 12.20) times and (80.10 ± 16.02) times, respectively], significantly higher than those in the normal group (49.00 ± 10.05) (P <0.01) In the test, the latency of the first day of positioning navigation training was significantly shorter than that of the normal group and the stimulation group [(13.12 ± 2.27) s, (27.34 ± 5.00) s, (30.62 ± 4.54) s] (P <0.05) (P <0.05). After 10 weeks, the body weight of the drug group and the normal group [(24.60 ± 0.43) g, (26.60 ± 0.43) g, ± 0.62) g] (P <0.05). CONCLUSION: Long-term chronic low-dose caffeine injection can slow the body weight gain of mice under chronic stimulation and at the same time enhance the spatial learning and memory abilities of mice under stimulation. Long-term chronic stimulation can also enhance the exploration of mice.