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目的 测定卵巢上皮性癌 (OEC)患者血清和腹腔液中VEGF蛋白水平 ,并探讨它的诊断价值。方法 采用酶链免疫吸附试验检测 2 8例OEC患者血清和腹腔液中VEGF蛋白水平 ,利用ROC曲线分析灵敏度和特异性。结果 ①OEC组血清VEGF蛋白水平分别显著高于健康志愿组 (中位数 4 6 3 2ng/Lvs 194 5ng/L ,P <0 0 5 )及良性肿瘤组 (中位数 4 6 3 2ng/Lvs 2 0 4 1ng/L ,P <0 0 1) ,良性肿瘤组与健康志愿组之间无显著性差异 (P >0 0 5 ) ;OEC组腹腔液VEGF蛋白水平极显著高于良性肿瘤组 (中位数为 2 6 30 5ng/Lvs 2 4 1 1ng/L ,P <0 0 0 1)。②ROC曲线显示 ,OEC血清VEGF测定特异性在 6 0 %~ 70 %范围内 ,其灵敏度可达 70 %~ 80 % ;OEC腹腔液VEGF测定特异性在 90 %~ 10 0 %范围内 ,其灵敏度可达 90 %以上。结论 OEC患者血清和腹腔液VEGF水平显著升高 ;血清和腹腔液VEGF测定 ,尤其是腹腔液VEGF ,可能有望成为OEC诊断的辅助性指标
Objective To determine the level of VEGF protein in serum and peritoneal fluid of patients with epithelial ovarian cancer (OEC) and to explore its diagnostic value. Methods Enzyme linked immunosorbent assay was used to detect the serum and peritoneal fluid VEGF levels in 28 patients with OEC. The sensitivity and specificity were analyzed by ROC curve. Results ① The levels of VEGF protein in OEC group were significantly higher than those in healthy volunteer group (median 4 632ng / Lvs 194 5ng / L, P <0 05) and benign tumor group (median 3632ng / Lvs 2 There was no significant difference between benign tumor group and healthy volunteer group (P> 0.05). The protein level of VEGF in peritoneal fluid of OEC group was significantly higher than that in benign tumor group (P <0.01) The median of 2 6 30 5 ng / Lvs 2 4 1 1 ng / L, P <0 0 0 1). The ROC curve showed that the specificity of VEGF in OEC serum was between 70% and 80% in the range of 60% -70%, and the specificity of VEGF in OEC peritoneal fluid was in the range of 90% -100% More than 90%. Conclusion The levels of VEGF in serum and peritoneal fluid of patients with OEC are significantly increased. The serum and peritoneal fluid VEGF levels, especially the peritoneal fluid VEGF, may be expected to be the auxiliary indicators for OEC diagnosis